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anti jam1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti jam1
    Anti Jam1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+jam1/JAM-A+Rabbit+mAb/pmc12966279-352-18-20
    Average 94 stars, based on 6 article reviews
    anti jam1 - by Bioz Stars, 2026-09
    94/100 stars

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    Incubation:

    Article Title: In vivo dual RNA-Seq uncovers key effectors of epithelial barrier disruption by an extracellular pathogen
    Article Snippet: At the indicated times, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature (RT) and subsequently incubated for 10 min in 0.5% saponin (Sigma) in PBS and for 1 h in 1% BSA (Sigma) and 0.075% saponin in PBS. .. The cells were incubated overnight at 4 °C with the anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-JAM1 (82196; Cell Signaling), anti-pan-cadherin (PA5-16766; ThermoFisher) or anti-connexin-43 (3512; Cell Signaling) antibodies at 1:100. .. Cells were washed and incubated for 1 h with Alexa Fluor 555 or 488 antibody (ThermoFisher) at 1:500.

    Article Title: In Vivo Dual RNA-Seq uncovers key toxin-like effectors of epithelial barrier disruption and tissue colonization by an extracellular bacterial pathogen
    Article Snippet: At the indicated times, the cells were fixed with 4% paraformaldehyde for 15 min at room temperature (RT) and subsequently incubated for 10 min in 0.5% saponin (Sigma) in PBS and for 1 hr in 1% BSA (Sigma) and 0.075% saponin in PBS. .. The cells were incubated overnight at 4°C with the anti-ZO1 (13663; Cell Signaling), anti-ZO2 (2847; Cell Signaling), anti-F-actin (PHDH1; Tebu), anti-JAM1 (82196; Cell Signaling) or anti-connexin-43 (3512; Cell Signaling) antibodies at 1:100. .. Cells were washed and incubated for 1 hr with Alexa Fluor 555 or 488 antibody (ThermoFisher) at 1:500.



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    Murine MSCs treated with indomethacin are more susceptible to phagocytosis by activated primary macrophages. A Experimental design: bone marrow-derived monocytes were differentiated into macrophages (Mφ) with M-CSF. Macrophages were then activated with LPS + IFN-γ to induce a pro-inflammatory M1-like phenotype. Activated macrophages were co-cultured in direct contact with control or indomethacin-treated MSCs. Macrophage phenotype was analyzed by flow cytometry (FACS), with all cells gated on the CD45 + CD11b + F4/80 + population, representing differentiated macrophages. B - C Median fluorescence intensity (MFI) of DiD, indicating phagocytosis, was measured in the pro-inflammatory macrophage subpopulations MHC-II + CD86 + ( B ) and MHC-II + CD80 + macrophages ( C ). Representative histograms are shown alongside MFI values. Data are shown as mean ± SD. n = 3 independent experiments. Statistical significance was assessed using one-way ANOVA. *: p < 0.05; **: p < 0.005; ***: p < 0.001; ****: p < 0.0001

    Journal: Cell Regeneration

    Article Title: Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration

    doi: 10.1186/s13619-025-00273-7

    Figure Lengend Snippet: Murine MSCs treated with indomethacin are more susceptible to phagocytosis by activated primary macrophages. A Experimental design: bone marrow-derived monocytes were differentiated into macrophages (Mφ) with M-CSF. Macrophages were then activated with LPS + IFN-γ to induce a pro-inflammatory M1-like phenotype. Activated macrophages were co-cultured in direct contact with control or indomethacin-treated MSCs. Macrophage phenotype was analyzed by flow cytometry (FACS), with all cells gated on the CD45 + CD11b + F4/80 + population, representing differentiated macrophages. B - C Median fluorescence intensity (MFI) of DiD, indicating phagocytosis, was measured in the pro-inflammatory macrophage subpopulations MHC-II + CD86 + ( B ) and MHC-II + CD80 + macrophages ( C ). Representative histograms are shown alongside MFI values. Data are shown as mean ± SD. n = 3 independent experiments. Statistical significance was assessed using one-way ANOVA. *: p < 0.05; **: p < 0.005; ***: p < 0.001; ****: p < 0.0001

    Article Snippet: Subsequently, cells were stained with specific antibodies targeting CD45 (clone 30-F11), CD11b (clone M1/70), CD80 (clone 16-10A1) (BD Biosciences), MHC-II (clone M5/114.15.2) (Miltenyi Biotec, Germany), and F4/80 (clone BM8) (eBioscience, USA).

    Techniques: Derivative Assay, Cell Culture, Control, Flow Cytometry, Fluorescence